Bioanalysis – Protein digestion enzymes

We have been formulating several protein digestion enzymes like trypsin, different proteases and peptidases for improved resistance to chaotropic conditions (high concentrations of urea, sonication, temperature). These are particularly relevant for the digestion and further characterization of complex and large proteins. We have recently successfully completed a proof of concept for the digestion of cross-linked proteins for the quantitative release of a biomarker.

Key benefits of our technology for protein digestion:

  • No self-digestion of shielded enzymes
  • No contribution of enzymes to the AA profile
  • Unpolluted peptide map or AA composition
  • Clean digest with detectable biomarker
  • Easy-to-use particles, easily removed from the AA mixture
  • Complete digestion of complex proteins
  • High reproducibility (85 %)
  • Transferable to CROs

Biotransformation enzymes

We have successfully demonstrated the added-value of our technology for enzyme-mediated bioconjugation or post-translational modifications of antibodies, and for the synthesis of building blocks using enzymes such as transaminase or glucosidase for example.

Key benefits of our technology for biotransformation:

  • No enzyme in the final product, particles easily removed
  • No leaching of enzymes
  • Resistance to organic solvents and to temperature
  • Improved space-time yields
  • Chirality Bioingredient enzymes: We have formulated the combination of glucose oxidase (GOX) and lactoperoxidase (LPO) for the safe use of their antimicrobial properties in personal care products.  Key benefits include:
  • Safety: no leaching of enzymes from the formulation
  • Activity: the enzyme remains active in its shield
  • Absence of immunogenicity: the enzyme is never in contact with the immune system, no antibody binding measured

Enzymes as active ingredients

Our enzymes can also be tailor-made for the detergent and personal care industry, increasing either the stability of stain removing enzymes and acting as actives in cosmetic formulations such as anti-aging or other benefit-providing ingredients.

Key benefits of our technology for consumer care:

  • Stabilization of the enzymes, avoiding digestion of certain enzymes by proteases
  • Increased storage stability
  • No leakage, ensuring the avoidance of immune reactions
  • Resistant to other ingredients
  • Remaining active although shielded

Contact us if you want to benefit from these formulated enzymes.